Effects of VOO, TO, and OA treatments on total G protein αi2, αi3 and αq/11 subunits and PKCβ1 levels in aorta. Sprague–Dawley rats received vehicle (V), VOO (2 g·kg−1), TO (1 g·kg−1), or OA (1 g·kg−1) p.o. every 12 h for 14 days. Insets show representative immunoblots of aortic G protein αi2 (A), G protein subunit αi3 (B), G protein subunit αq/11 subunit (C), and PLCβ1 (PLCβ1a + PLCβ1b) (D) after vehicle, VOO, TO, or OA administration. The amount of total protein loaded was 24 μg for Gαi2, αi3, and αq/11 subunits and 80 μg for PLCβ1. The columns represent the mean ± SEM (n = 5) of total protein levels quantified against standard curves and normalized to the protein content from vehicle-treated rats (taken as 100%). *, P < 0.05 and **, P < 0.01 vs. vehicle-treated rats (ANOVA).