Effects of VOO, TO, and OA
treatments on total G protein
αi2,
αi3 and
αq/11 subunits and
PKCβ1 levels in aorta. Sprague–Dawley rats
received vehicle (V), VOO (2 g·kg−1), TO (1
g·kg−1), or OA (1 g·kg−1) p.o. every
12 h for 14 days. Insets show representative immunoblots
of aortic G protein
αi2 (A), G
protein subunit
αi3 (B), G protein subunit
αq/11 subunit
(C), and
PLCβ1 (PLCβ1a + PLCβ1b) (D) after vehicle, VOO, TO, or OA administration. The
amount of total protein loaded was 24
μg for Gαi2,
αi3, and
αq/11 subunits and 80
μg for PLCβ1.
The columns represent the mean
± SEM (n = 5) of total protein levels quantified against
standard curves and normalized to the protein content from
vehicle-treated rats (taken as 100%). *, P < 0.05 and **, P < 0.01 vs.
vehicle-treated rats (ANOVA).