Fadul Allah Eltayeb

Biography:

Mr. Eltayeb obtained his MSc in Biochemistry, Sudan Academy of Science (2012). In Khartoum, he was Co-Operator Research Assistant at the Phytochemistry and Taxonomy Department, Medicinal & Aromatic Plants Research Institute, National Centre for Research. Formerly, Research Fellow at the Medical Biochemistry Research Unit, Medicinal & Aromatic Plants Research Institute, National Centre for Research. Currently, he is a Researcher, Medical Biochemistry Research Unit, Medicinal &Aromatic Plants Research Institute, National Centre for Research, Khartoum.

Abstract:

Many clinical disorders are closely associated with the immune system; free radicals or Reactive Oxygen Species (ROS) appear to be associated with a number of human neurodegenerative disorders, inflammation, diabetes, viral infections, autoimmune pathologies and digestive system disorders. Previously, it was suggested that many therapeutic effects of plant extracts are due to the influence on the immune system. In the present work, barks and leaves of Lawsonia inermis LI, commonly used in Sudanese traditional medicines against infectious diseases were extracted by cold extraction methodology using methanol and chloroform. The plant extracts, which were obtained subjected for investigation of antioxidant activity and cytotoxic effect using DPPH, iron chelating assays and cell viability of Polymorph Nuclear Cells (PMNs) and Mono Nuclear Cells (MNCs) by using trepan blue. Results obtained from this study showed that potential iron chelating and Radical Scavenging Activity (RSA) of leaves and barks of Lawsonia interims LI. metabolic extract against ferrous iron and DPPH free radical, which were found to be (95%, 92% on LIL) / (52%, 50% on LIB) at a concentration 500µg/mL respectively, and possess inhibitory effects in the range of 64.3%, 74.6% on PMNs and 40.9%, 75.4% on MNCs respectively at a concentration 250µg/mL. Chloroform extracts of LI (leave and bark) were exhibited a high effect (68.2%–74.1%) on PMNs, while the mortality on MNCs was found to be 79.8% and 92.2% respectively.