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Diagnosis:
(This applies to both PI3 and BRSV) 4-fold rise in ELISA antibody in paired serum samples from several animals. Virus isolation early in disease. IF on nasal aspirates or bronchial epithelium. Control: Correct ventilation and stocking density; avoid mixing different ages from different locations in same airspace. Current vaccines utilise a temperature sensitive mutant which gives local protection after intranasal inoculation at 34OC and does not replicate at 370C. |